Doktorandin Doktorand PhD student Biomedical Research Laboratory

The position with the project focus Patient-Derived Thyroid Cancer Organoids from Advanced Thyroid cancers as a Platform to Guide Precision Therapy is to be filled in the Biomedical Research Laboratory in Medical Clinic 1 by March 1 2026.

The Biomedical Research Laboratory of the Medical Clinic 1 at the University Hospital Frankfurt is headed by Prof. Angela Brieger. The Medical Clinic 1 is directed by Prof. Stefan Zeuzem. The advised research project is led by Dr. Viktoria Köhler whose research focuses on the molecular and clinical aspects of advanced thy-roid cancer.

Advances in genomic profiling have identified key driver alterations in thyroid cancer; however translating these molecular insights into individualized therapeutic strategies remains a significant challenge. Patient-derived tumor organoids (PDTOs) have emerged as a powerful platform for translational cancer research faithfully recapitulating histopathological architecture as well as essential genomic features relevant for bio-logical studies and clinical decision-making.

Drug screening in PDTOs enables systematic evaluation of approved tyrosine kinase inhibitors investiga-tional compounds and rational drug combinations. Nevertheless current thyroid cancer organoid models are predominantly epithelial and lack essential components of the tumor microenvironment (TME) including can-cer-associated fibroblasts (CAFs) immune cell populations extracellular matrix complexity and vascular structures. Incorporating TME elements and vascular networks through co-culture approaches and microfluid-ic organ-on-chip technologies represents a promising next-generation strategy to enhance physiological relevance.

As part of the advertised PhD project the candidate will establish and characterize a comprehensive PDTO biobank. Additional responsibilities include performing high-throughput drug sensitivity screening to identify patient-specific therapeutic vulnerabilities integrating TME components vascularization and tissue dynamics into organoid models and correlating organoid drug responses with corresponding clinical outcomes.

The position is limited to 12 months and involves 26 hours of work per week.

Your Role:

Main techniques and analysis for this project are:

• Cultivation transfection and analysis of human cell lines
• Establishment cultivation and maintenance of PDTOs and 3D co-culture systems
• Protein and nucleic acid isolation from cell tissue patient tissue and organoids
• Molecular analyses including qPCR sequenc-ing-based assays and western blot analysis
• Histological and immunohistochemical staining
• Development and optimization of drug screening assays
• Quantification and analysis of imaging da-tasets organoid growth kinetics and treatment responses
• Statistical analysis and evaluation

• You have completed a university degree (master/diploma) in the fields of biology human biology molecular medicine biochemistry veterinary medicine or comparable.
• Ideally you have prior experience with 3D cell culture systems organoid technologies co-culture models microfluidic or organ-on-chip platforms as well as high-throughput screening methods.
• You are reliable committed a team player and have a high level of self-motivation
• You have good written and spoken English skills.
• Due to legal regulations valid proof of measles immunity / measles vaccination is required

• In addition to an attractive salary based on acollective agreement (TV-G-U E-13 65%) with an annual special payment you benefit from long-term security through company pension schemes
• Internal and external training for your professional development
• Benefit from our attractive health offers. We offer regular online and face-to-face courses on nutrition relaxation sports and exercise
• Part-time employment is possible we offer childcare in our daycare center (if you have any questions please contactUKF-Familienservice) child care during holidays
• Free public transport in all Hessen (Free State Ticket Hessen)
• Uniklinik-Campus Mensa Cafés
• Insights: Instagram YouTube LinkedIn
• FAQs for new employees

Contact: Dr. Viktoria Köhler
Phone: 0151-

Application Deadline: 13.02.2026

We welcome applications from candidates of all genders. Women are currently underrepresented in these positions at Universitätsmedizin Frankfurt. Therefore applications from women are especially encouraged. Applicants with severe disabilities will be given preferential consideration in cases of equal personal and professional qualifications.