Research Scientist - High Throughput Molecular Biology and Expression (m/w/d)
Hofmann Personal
Wuppertal
Ihre Aufgaben
- Test and establish a high throughput cloning and expression workflow for non-antibody proteins.
- Implement high throughput DNA assembly techniques.
- Evaluate and establish high throughput compatible protein expression and quantification methods for E. coli and HEK as standard expression hosts.
- Collaborate closely with experts in molecular biology, data science, expression technologies, protein purification, and lab automation.
Ihre Qualifikation :
- Master s degree with at least 4 years of relevant experience or a PhD with at least 2 years of relevant experience in molecular biology, protein engineering, biotechnology, or protein biochemistry.
- Hands-on knowledge in generating DNA constructs in high throughput.
- Independent development of cloning strategies and proficiency in standard sequencing and molecular biology software packages (e.
g., Geneious, Sequencer).
- In-depth and hands-on knowledge of state-of-the-art high throughput molecular biology cloning technologies (e.g., Golden Gate, Gibson, SLIC, site-directed mutagenesis & sequence analysis).
- Experience in structure-guided protein engineering and biophysical protein characterization using standard software packages (e.
g., AlphaFold, ChimeraX / PyMOL, MPI Bioinformatics Toolkit, SIB Protein Toolkit).
- Hands-on experience with expression in standard organisms (E. coli, HEK, CHO).
- Proficiency in standard protein biochemistry techniques (SDS-PAGE, Native PAGE, cGE, SEC-MALS, Western Blot).
- Experience in multi-step protein purification strategies and their optimization.
- Preferred experience with automated systems (e.g., Hamilton Vantage).
Ihr Vorteil :
- Quick and uncomplicated entry
- Cross-qualification entry opportunities
- Above-tariff pay
- Wage increase through collectively agreed industry surcharges
- Holiday and Christmas bonus
- Free personal protective equipment
- Opportunity to be taken on by our customers
- Occupational health and safety support
Vor 30+ Tagen