Research Scientist - High Throughput Molecular Biology and Expression (m/w/d)

Hofmann Personal
Wuppertal
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Ihre Aufgaben

  • Test and establish a high throughput cloning and expression workflow for non-antibody proteins.
  • Implement high throughput DNA assembly techniques.
  • Evaluate and establish high throughput compatible protein expression and quantification methods for E. coli and HEK as standard expression hosts.
  • Collaborate closely with experts in molecular biology, data science, expression technologies, protein purification, and lab automation.

Ihre Qualifikation :

  • Master s degree with at least 4 years of relevant experience or a PhD with at least 2 years of relevant experience in molecular biology, protein engineering, biotechnology, or protein biochemistry.
  • Hands-on knowledge in generating DNA constructs in high throughput.
  • Independent development of cloning strategies and proficiency in standard sequencing and molecular biology software packages (e.

g., Geneious, Sequencer).

  • In-depth and hands-on knowledge of state-of-the-art high throughput molecular biology cloning technologies (e.g., Golden Gate, Gibson, SLIC, site-directed mutagenesis & sequence analysis).
  • Experience in structure-guided protein engineering and biophysical protein characterization using standard software packages (e.

g., AlphaFold, ChimeraX / PyMOL, MPI Bioinformatics Toolkit, SIB Protein Toolkit).

  • Hands-on experience with expression in standard organisms (E. coli, HEK, CHO).
  • Proficiency in standard protein biochemistry techniques (SDS-PAGE, Native PAGE, cGE, SEC-MALS, Western Blot).
  • Experience in multi-step protein purification strategies and their optimization.
  • Preferred experience with automated systems (e.g., Hamilton Vantage).

Ihr Vorteil :

  • Quick and uncomplicated entry
  • Cross-qualification entry opportunities
  • Above-tariff pay
  • Wage increase through collectively agreed industry surcharges
  • Holiday and Christmas bonus
  • Free personal protective equipment
  • Opportunity to be taken on by our customers
  • Occupational health and safety support
  • Vor 30+ Tagen
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